Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 41
Page 1-3
... origin of DNA replication . Usually , a plasmid will contain only one origin of replication together with its associated cis - acting control elements ( the whole genetic unit being defined as a " replicon " ) . Very rarely , however ...
... origin of DNA replication . Usually , a plasmid will contain only one origin of replication together with its associated cis - acting control elements ( the whole genetic unit being defined as a " replicon " ) . Very rarely , however ...
Page 3-19
... origin of replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency with which cosmid DNA can be packaged in vivo into transducing particles . The only useful cloning site in pHC79 ...
... origin of replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency with which cosmid DNA can be packaged in vivo into transducing particles . The only useful cloning site in pHC79 ...
Page 4-44
... origin of replication ( derived from p15A [ Selzer et al . 1983 ] ) , and the kanamycin resistance gene from Tn903 ( Grindley and Joyce 1980 ) at the Aval site ( nucleotide 5825 of bacteriophage M13 ) ( Figure 4.7 ) . When M13KO7 ...
... origin of replication ( derived from p15A [ Selzer et al . 1983 ] ) , and the kanamycin resistance gene from Tn903 ( Grindley and Joyce 1980 ) at the Aval site ( nucleotide 5825 of bacteriophage M13 ) ( Figure 4.7 ) . When M13KO7 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml