Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-98
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Preparation of Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro Packaging extracts are available at reasonable cost from commercial sources . These work ...
Page 2-104
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage λ DNA . ii . These extracts exhibit a high ...
... packaging reactions are stored in closed tubes at 4 ° C . Notes i . Each batch of extracts should be tested for packaging efficiency with a standardized preparation of intact bacteriophage λ DNA . ii . These extracts exhibit a high ...
Page 3-33
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
... packaging extracts ( Bates and Swift 1983 ) . Extracts used to package cosmids should be prepared using a buffer that contains spermidine but not putrescine . When putrescine is omitted from the packaging extract and the packaging ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml