Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 59
Page 1-25
... pellet as dry as possible . The supernatant can be conveniently removed with a disposable pipette tip attached to a vacuum line ( see Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface of the liquid . Keep the tip as ...
... pellet as dry as possible . The supernatant can be conveniently removed with a disposable pipette tip attached to a vacuum line ( see Figure 1.3 ) . Use a gentle vacuum and touch the tip to the surface of the liquid . Keep the tip as ...
Page 7-7
... pellet three times by resuspension in 10 volumes of ice - cold PBS lacking calcium and magnesium ions ; use a wide- bore pipette to disperse the cell pellet gently , but completely , each time . b . Estimate the volume of the packed ...
... pellet three times by resuspension in 10 volumes of ice - cold PBS lacking calcium and magnesium ions ; use a wide- bore pipette to disperse the cell pellet gently , but completely , each time . b . Estimate the volume of the packed ...
Page 7-62
... pellet with 70 % ethanol , and recentrifuge . Care- fully remove all of the ethanol , and store the pellet of nucleic acids at room temperature until the last visible traces of ethanol have evapo- rated . Do not allow the pellet to ...
... pellet with 70 % ethanol , and recentrifuge . Care- fully remove all of the ethanol , and store the pellet of nucleic acids at room temperature until the last visible traces of ethanol have evapo- rated . Do not allow the pellet to ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml