Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-4
... plasmids is caused by inefficient binding of truncated RNA I to RNA II . In addition to controlling copy number , the regions of the plasmid encoding RNA I , RNA II , and the Rop protein also determine whether two different plasmids ...
... plasmids is caused by inefficient binding of truncated RNA I to RNA II . In addition to controlling copy number , the regions of the plasmid encoding RNA I , RNA II , and the Rop protein also determine whether two different plasmids ...
Page 1-5
... Plasmids like PSC101 are therefore under stringent replicative control and are present at only 5 copies per cell or less . Because stringently controlled replication requires ongoing expression of a plasmid - encoded protein , the copy ...
... Plasmids like PSC101 are therefore under stringent replicative control and are present at only 5 copies per cell or less . Because stringently controlled replication requires ongoing expression of a plasmid - encoded protein , the copy ...
Page 1-7
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml