Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xi
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Contents BOOK 1 Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Contents BOOK 1 Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 ...
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A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Plasmid Vectors DEVELOPMENT OF PLASMID CLONING VECTORS The development of plasmid vectors has proceeded in three phases : 1. Selectable markers were introduced into plasmids ...
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... Plasmid Vectors In principle , cloning in plasmid vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform ...
... Plasmid Vectors In principle , cloning in plasmid vectors is very straightforward . The plasmid DNA is cleaved with a restriction enzyme and joined in vitro to foreign DNA . The resulting recombinant plasmids are then used to transform ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml