Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 82
Page 1-92
... plates . The colonies are consolidated on a master agar plate and on a nitrocellulose filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C ...
... plates . The colonies are consolidated on a master agar plate and on a nitrocellulose filter laid on the surface of a second agar plate . After a period of growth , the colonies are lysed in situ . The master plate is stored at 4 ° C ...
Page 2-65
... plate containing 30-35 ml of hardened bottom agar . A freshly poured plate equilibrated to room temperature gives the best results , but an older plate ( 1–4 days old ) gives a satisfactory yield . Adequate titers of most strains of ...
... plate containing 30-35 ml of hardened bottom agar . A freshly poured plate equilibrated to room temperature gives the best results , but an older plate ( 1–4 days old ) gives a satisfactory yield . Adequate titers of most strains of ...
Page 6-41
... plate , lay the plate on a pad of paper in a fume hood and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate in ...
... plate , lay the plate on a pad of paper in a fume hood and pour a small quantity of siliconizing fluid ( e.g. , Sigmacote ) onto it . Wipe the fluid over the surface of the plate with a pad of Kimwipes , and then rinse the plate in ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml