Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-35
... polycloning sites . These bac- teriophages have bacteriophage T7 and T3 promoters in the polycloning sites . Thus , RNA probes can be synthesized without subcloning , and chromosome walking is made easier . λ2001 A2001 contains multiple ...
... polycloning sites . These bac- teriophages have bacteriophage T7 and T3 promoters in the polycloning sites . Thus , RNA probes can be synthesized without subcloning , and chromosome walking is made easier . λ2001 A2001 contains multiple ...
Page 2-37
... polycloning - site sequences are inverted with respect to one another . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites . The bacteriophage T3 and T7 promoters adjacent to the polycloning sites allow RNA ...
... polycloning - site sequences are inverted with respect to one another . Inserts that destroy the XhoI or BamHI site can be excised at flanking sites . The bacteriophage T3 and T7 promoters adjacent to the polycloning sites allow RNA ...
Page 4-33
... polycloning site of the vector is cleaved with a single restriction enzyme , and the resulting linear DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background of ...
... polycloning site of the vector is cleaved with a single restriction enzyme , and the resulting linear DNA is ligated with an excess of foreign DNA that has compatible termini . No effort is made to reduce the background of ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml