Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 90
Page xxiv
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page xxv
... Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING ABSORBED PROBES 10.40 10.38 SUBTRACTED LIBRARIES 10.40 SUBTRACTED PROBES ...
... Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING ABSORBED PROBES 10.40 10.38 SUBTRACTED LIBRARIES 10.40 SUBTRACTED PROBES ...
Page 7-66
... Probes Single - stranded DNA probes are prepared by one of two methods : • Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are ...
... Probes Single - stranded DNA probes are prepared by one of two methods : • Separation of the strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml