Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page viii
... protocols . We also strongly recommend reading the relevant protocols in their entirety before commencing work . This allows reagents to be prepared ahead of time and permits the investigator to carry out the protocol efficiently . This ...
... protocols . We also strongly recommend reading the relevant protocols in their entirety before commencing work . This allows reagents to be prepared ahead of time and permits the investigator to carry out the protocol efficiently . This ...
Page 2-64
... protocols work equally well . Protocol II is more rapid , but it requires additional manipulations . In Protocol I , the bacteriophages are eluted from the plate by diffusion into SM , whereas in Protocol II , the bacteriophages are ...
... protocols work equally well . Protocol II is more rapid , but it requires additional manipulations . In Protocol I , the bacteriophages are eluted from the plate by diffusion into SM , whereas in Protocol II , the bacteriophages are ...
Page 2-98
... protocols available for the preparation of packaging extracts , the two presented below are the simplest to perform and are highly efficient . Protocol I is representative of the classical type of packaging in that two lysogens that ...
... protocols available for the preparation of packaging extracts , the two presented below are the simplest to perform and are highly efficient . Protocol I is representative of the classical type of packaging in that two lysogens that ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml