Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 86
Page 1-63
... reaction conditions . Ligation of one end of DNA to another can be regarded as a bimolecular reaction whose velocity under standard conditions is determined solely by the concentration of compatible DNA termini . This is true no matter ...
... reaction conditions . Ligation of one end of DNA to another can be regarded as a bimolecular reaction whose velocity under standard conditions is determined solely by the concentration of compatible DNA termini . This is true no matter ...
Page 5-36
... reaction , since it alone has a 5 ' - 3 ' exonuclease activity that can remove nucleotides from the DNA strand ahead of the advancing enzyme . 2. The holoenzyme was originally used for synthesis of the second strand of cDNA in cDNA ...
... reaction , since it alone has a 5 ' - 3 ' exonuclease activity that can remove nucleotides from the DNA strand ahead of the advancing enzyme . 2. The holoenzyme was originally used for synthesis of the second strand of cDNA in cDNA ...
Page 5-44
... reaction to overwhelm the powerful 3'5 ' exonuclease activity . 2. End - labeling of DNA molecules with protruding 3 ' tails . This reaction works in two stages . First , the potent 3'5 ' exonuclease activity removes protruding 3 ...
... reaction to overwhelm the powerful 3'5 ' exonuclease activity . 2. End - labeling of DNA molecules with protruding 3 ' tails . This reaction works in two stages . First , the potent 3'5 ' exonuclease activity removes protruding 3 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml