Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 27
Page 3-5
... cosmids carry an origin of replication ( usually the ColE1 origin ) and a ... recombinant DNA is injected into the cell , where it circularizes ( via the ... recombinant cosmids can therefore be selected using media containing the ...
... cosmids carry an origin of replication ( usually the ColE1 origin ) and a ... recombinant DNA is injected into the cell , where it circularizes ( via the ... recombinant cosmids can therefore be selected using media containing the ...
Page 3-44
... Cosmid Libraries Cosmids packaged in bacteriophage à particles are stable for several weeks when stored in SM containing a few drops of chloroform . If sufficient recombinant cosmids are obtained in the original packaging reactions ...
... Cosmid Libraries Cosmids packaged in bacteriophage à particles are stable for several weeks when stored in SM containing a few drops of chloroform . If sufficient recombinant cosmids are obtained in the original packaging reactions ...
Page 3-45
... Cosmid Libraries Cosmids packaged in bacteriophage A particles are stable for several weeks when stored in SM containing a few drops of chloroform . If sufficient recombinant cosmids are obtained in the original packaging reactions ...
... Cosmid Libraries Cosmids packaged in bacteriophage A particles are stable for several weeks when stored in SM containing a few drops of chloroform . If sufficient recombinant cosmids are obtained in the original packaging reactions ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml