Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 78
Page 1-28
... remove any adherent droplets of fluid . 9. Rinse the pellet of double - stranded DNA with 1 ml of 70 % ethanol at 4 ° C . Remove the supernatant as described in step 8 , and allow the pellet of nucleic acid to dry in the air for 10 ...
... remove any adherent droplets of fluid . 9. Rinse the pellet of double - stranded DNA with 1 ml of 70 % ethanol at 4 ° C . Remove the supernatant as described in step 8 , and allow the pellet of nucleic acid to dry in the air for 10 ...
Page 1-29
... Remove the supernatant by gentle aspiration . Stand the tube in an inverted position on a paper towel to allow all of the fluid to drain away . Remove any drops of fluid adhering to the walls of the tube . The supernatant can be ...
... Remove the supernatant by gentle aspiration . Stand the tube in an inverted position on a paper towel to allow all of the fluid to drain away . Remove any drops of fluid adhering to the walls of the tube . The supernatant can be ...
Page 2-83
... Remove two aliquots ( 0.2 μg each ) of the undigested DNA and store on ice . 3. Add a threefold excess ( 75-150 units ) of the appropriate restriction enzyme and incubate for 1 hour at the optimal temperature recommended by the ...
... Remove two aliquots ( 0.2 μg each ) of the undigested DNA and store on ice . 3. Add a threefold excess ( 75-150 units ) of the appropriate restriction enzyme and incubate for 1 hour at the optimal temperature recommended by the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml