Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-40
... room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 ...
... room temperature in a Sorvall SS34 rotor ( or its equivalent ) . 3. Decant the supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 ...
Page 4-29
... room temperature . The solution of polyethylene glycol in 2.5 M NaCl should be sterilized by filtration . It may then be stored indefinitely at room temperature . 4. Recover the precipitated bacteriophage particles by centrifugation at ...
... room temperature . The solution of polyethylene glycol in 2.5 M NaCl should be sterilized by filtration . It may then be stored indefinitely at room temperature . 4. Recover the precipitated bacteriophage particles by centrifugation at ...
Page 6-30
... temperature Agarose Gels A number of types of agarose are available in which hydroxyethyl groups have been ... room temperature , transfer it and its supporting glass plate to a horizontal surface in a cold room to ensure that the gel ...
... temperature Agarose Gels A number of types of agarose are available in which hydroxyethyl groups have been ... room temperature , transfer it and its supporting glass plate to a horizontal surface in a cold room to ensure that the gel ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml