Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-13
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
... sequences . The final genetic factor to consider in choosing a vector is the presence of a chi site . Wild - type bacteriophage A contains no chi site , but mutants carrying an octanucleotide chi sequence were detected as suppressors of ...
Page 5-16
... sequences : GTCGAC GTCAAC GTTGAC GTTAA C The M.TaqI methylase recognizes only the sequence TCGA and methylates the adenine residue ( McClelland 1981 ) . The subset of HincII recognition sequences that contains the internal sequence TCGA ...
... sequences : GTCGAC GTCAAC GTTGAC GTTAA C The M.TaqI methylase recognizes only the sequence TCGA and methylates the adenine residue ( McClelland 1981 ) . The subset of HincII recognition sequences that contains the internal sequence TCGA ...
Page 5-50
... sequencing ( Chapter 13 ) and to amplify specific sequences of DNA in vitro by the polymerase chain reaction ( Chapter 14 ) . This is accomplished using two oligonucleotide primers complementary to sequences that flank the region of ...
... sequencing ( Chapter 13 ) and to amplify specific sequences of DNA in vitro by the polymerase chain reaction ( Chapter 14 ) . This is accomplished using two oligonucleotide primers complementary to sequences that flank the region of ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml