Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 85
Page 1-28
... step 3 , page 1.25 , or step 8 above . In this case , extract the final DNA preparation with phenol : chloroform and reprecipitate the DNA with ethanol . Frequently , difficulties in cleavage with restriction enzymes can be overcome by ...
... step 3 , page 1.25 , or step 8 above . In this case , extract the final DNA preparation with phenol : chloroform and reprecipitate the DNA with ethanol . Frequently , difficulties in cleavage with restriction enzymes can be overcome by ...
Page 1-44
... step 2. The tip of the pipette should touch the bottom of the tube . Carefully load the clear , red solution ( from beneath the scum ) prepared in step 7 into the tube through the pipette so that the sample is layered under the 1.47 g ...
... step 2. The tip of the pipette should touch the bottom of the tube . Carefully load the clear , red solution ( from beneath the scum ) prepared in step 7 into the tube through the pipette so that the sample is layered under the 1.47 g ...
Page 2-78
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. GLYCEROL STEP GRADIENT The following method is modified from Vande Woude et al . ( 1979 ) . 1. Follow the " Standard Method for Purification of Bacteriophage X " through step ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. GLYCEROL STEP GRADIENT The following method is modified from Vande Woude et al . ( 1979 ) . 1. Follow the " Standard Method for Purification of Bacteriophage X " through step ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml