Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 59
Page 4-24
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
... sterile tube ( 13 mm × 100 mm ) . 2. Touch the surface of the chosen plaque with the end of a sterile , 8 - cm - long wooden stick ( sometimes called applicator or orange stick ) . Immediately immerse the end of the stick in the LB ...
Page 7-26
... sterile Dispocolumn ( Bio - Rad ) ( or a pasteur pipette , plugged with sterile glass wool that has been treated with diethyl pyrocarbonate [ DEPC ] and autoclaved [ see page 7.3 ] ) . Wash the column with 3 column volumes of sterile ...
... sterile Dispocolumn ( Bio - Rad ) ( or a pasteur pipette , plugged with sterile glass wool that has been treated with diethyl pyrocarbonate [ DEPC ] and autoclaved [ see page 7.3 ] ) . Wash the column with 3 column volumes of sterile ...
Page 7-27
... sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect the eluate . Heating the RNA disrupts regions of secondary structure that might involve the ...
... sterile tube . When all of the RNA solution has entered the column , add 1 column volume of 1 × column - loading buffer and continue to collect the eluate . Heating the RNA disrupts regions of secondary structure that might involve the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml