Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 73
Page 2-68
... stored at 4 ° C in SM containing a small amount of chloroform ( 0.3 % ) . However , many workers have found that some bacteriophage A recombinants lose viability when stored in this way for a few months . This problem can usually be ...
... stored at 4 ° C in SM containing a small amount of chloroform ( 0.3 % ) . However , many workers have found that some bacteriophage A recombinants lose viability when stored in this way for a few months . This problem can usually be ...
Page 4-24
... stored for long periods of time or that will be used to make master stocks of valuable recombinants . 3. Let the suspension stand for 1 to 2 hours at room temperature to allow the bacteriophage particles to diffuse out of the agar . An ...
... stored for long periods of time or that will be used to make master stocks of valuable recombinants . 3. Let the suspension stand for 1 to 2 hours at room temperature to allow the bacteriophage particles to diffuse out of the agar . An ...
Page 7-18
... stored at -70 ° C . Cells grown in monolayers should be washed once with PBS , drained well , and either used immediately or quick - frozen in liquid nitrogen and stored at -70 ° C . This can be achieved as follows : 1. Make a shallow ...
... stored at -70 ° C . Cells grown in monolayers should be washed once with PBS , drained well , and either used immediately or quick - frozen in liquid nitrogen and stored at -70 ° C . This can be achieved as follows : 1. Make a shallow ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml