Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 97
Page 2-55
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
... strains of E. coli that have been designed to serve as specific hosts . Table 2.2 provides descriptions of some of the genetic markers found in these strains . The strains themselves are listed in Table 2.3 and are more fully described ...
Page 2-57
... Strains supE supF hflA recA recB recC sbcA sbc B recD lacl lacZ hsdR hsdS hsdM mcrB lon rpsL dam tonA Tn10 PMC9 Bacterial genes encoding suppressor tRNAs for amber mutations . supE inserts a glutamine residue at the codon UAG ; supF ...
... Strains supE supF hflA recA recB recC sbcA sbc B recD lacl lacZ hsdR hsdS hsdM mcrB lon rpsL dam tonA Tn10 PMC9 Bacterial genes encoding suppressor tRNAs for amber mutations . supE inserts a glutamine residue at the codon UAG ; supF ...
Page 4-13
... strains carrying this mutation will be modified and therefore protected against restriction if the vector is sub- sequently introduced into an hsd strain of E. coli K. • recA1 : The recA gene of E. coli codes for a DNA - dependent ...
... strains carrying this mutation will be modified and therefore protected against restriction if the vector is sub- sequently introduced into an hsd strain of E. coli K. • recA1 : The recA gene of E. coli codes for a DNA - dependent ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml