Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 77
Page 4-2
... strand • Site - directed mutagenesis using synthetic oligonucleotides TRANSCRIPTION P > P > P > Transcription terminator intergenic region ori ( + ) strand ( - ) strand P IV P > Bacteriophage M13 Wild Type ( 6.4 kb ) IX VIII VII ...
... strand • Site - directed mutagenesis using synthetic oligonucleotides TRANSCRIPTION P > P > P > Transcription terminator intergenic region ori ( + ) strand ( - ) strand P IV P > Bacteriophage M13 Wild Type ( 6.4 kb ) IX VIII VII ...
Page 5-20
... strand of the hemimethylated se- quence GGm CC / GGCC . Fnu EI Fnu4HI GATC GCNGC Gm6ATC ? ? m5 FokI GGATG ( 5 ... strand . EcoB is a type I restriction enzyme . complementary strand . m6T represents a 6 - methyladenine in the m6T ...
... strand of the hemimethylated se- quence GGm CC / GGCC . Fnu EI Fnu4HI GATC GCNGC Gm6ATC ? ? m5 FokI GGATG ( 5 ... strand . EcoB is a type I restriction enzyme . complementary strand . m6T represents a 6 - methyladenine in the m6T ...
Page 7-66
... strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are prepared by using restriction enzymes , singly or in combination , to generate a DNA fragment ...
... strands of a fragment of double - stranded DNA • De novo synthesis of a strand from a single - stranded template Strand - separated probes are prepared by using restriction enzymes , singly or in combination , to generate a DNA fragment ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml