Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 53
Page 1-39
... supernatant as possible to a fresh bottle . Discard the viscous liquid remaining in the centrifuge bottle . The failure to form a compact pellet is usually a consequence of inadequate mixing of the bacterial lysate with Solution III ...
... supernatant as possible to a fresh bottle . Discard the viscous liquid remaining in the centrifuge bottle . The failure to form a compact pellet is usually a consequence of inadequate mixing of the bacterial lysate with Solution III ...
Page 1-40
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
... supernatant carefully , and invert the open tube to allow the last drops of supernatant to drain away . Rinse the pellet and the walls of the tube with 70 % ethanol at room temperature . Drain off the ethanol , and use a pasteur pipette ...
Page 4-29
... supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual supernatant . A tiny pellet of precipitated bac- teriophage particles should be visible at the ...
... supernatant using a disposable pipette tip as described on pages 4.27-4.28 . Recentrifuge the tube for 30 seconds and remove any residual supernatant . A tiny pellet of precipitated bac- teriophage particles should be visible at the ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml