Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 97
Page xiv
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 Bacteriophage A Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE Preparation of Plating ...
... BACTERIOPHAGE VECTORS 2.55 Restriction and Modification 2.55 Amber Suppressors 2.55 Recombination Systems 2.55 Bacteriophage A Growth , Purification , and DNA Extraction 2.60 PLAQUE PURIFICATION OF BACTERIOPHAGE Preparation of Plating ...
Page 2-95
... bacteriophage à DNA in vitro was initially developed by Becker and Gold ( 1975 ) using mixtures of extracts prepared from bacteria infected with bacteriophage à mutant in genes required for assembly of bacteriophage particles . The ...
... bacteriophage à DNA in vitro was initially developed by Becker and Gold ( 1975 ) using mixtures of extracts prepared from bacteria infected with bacteriophage à mutant in genes required for assembly of bacteriophage particles . The ...
Page 5-58
... Bacteriophage SP6 and Bacteriophages T7 and T3 RNA Polymerases ( Bacteriophage SP6 - infected Salmonella typhimurium LT2 and bacteriophage T7- or T3 - infected E. coli ) USES Bacteriophage SP6 synthesizes a DNA - dependent RNA ...
... Bacteriophage SP6 and Bacteriophages T7 and T3 RNA Polymerases ( Bacteriophage SP6 - infected Salmonella typhimurium LT2 and bacteriophage T7- or T3 - infected E. coli ) USES Bacteriophage SP6 synthesizes a DNA - dependent RNA ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml