Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 87
Page 1-64
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , i = 2 N M × 10-3 ends / ml N ̧M where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when ji , the end ...
... termini in the solution . For double- stranded DNA with self - complementary cohesive termini , i = 2 N M × 10-3 ends / ml N ̧M where N is Avogadro's number and M is the molar concentration of the DNA . Theoretically , when ji , the end ...
Page 2-90
... termini of the vector be reannealed and ligated before treatment with phosphatase ; otherwise , concatemerization will be inhibited and the packaging efficiency of the DNA will be reduced signifi- cantly . 1. Ligate the cohesive termini ...
... termini of the vector be reannealed and ligated before treatment with phosphatase ; otherwise , concatemerization will be inhibited and the packaging efficiency of the DNA will be reduced signifi- cantly . 1. Ligate the cohesive termini ...
Page 5-13
... termini allows as many as 70 % of the combinations of termini created by different restriction enzymes to be joined . Two empirical approaches for determining whether any two protruding 5 ' termini can be ligated have been reported ...
... termini allows as many as 70 % of the combinations of termini created by different restriction enzymes to be joined . Two empirical approaches for determining whether any two protruding 5 ' termini can be ligated have been reported ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
47 other sections not shown
Other editions - View all
Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml