Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 79
Page xxiv
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
... TRANSFER OF DNA FROM AGAROSE GELS TO SOLID SUPPORTS 9.34 Transfer of DNA to Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO ...
Page 2-110
... transfer of bacteriophage DNA occurs quickly . Therefore , do not move the filter once contact with the plate is made . The easiest way to place the filter on the plate is to hold it by its edges , bending it slightly so that the middle ...
... transfer of bacteriophage DNA occurs quickly . Therefore , do not move the filter once contact with the plate is made . The easiest way to place the filter on the plate is to hold it by its edges , bending it slightly so that the middle ...
Page 7-46
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
... transfer , or electroblotting ( see Chapter 9 , pages 9.34-9.37 , for a discussion of the relative merits of these techniques ) . Capillary elution is carried out as described below ; vacuum transfer and electroblotting should be ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml