Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-74
... transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can be increased ...
... transformation of different bacterial strains by plasmids . Bac- teria treated according to the original protocol of Mandel and Higa yield 105-106 transformed colonies / μg of supercoiled plasmid DNA . This efficiency can be increased ...
Page 1-75
... transformation were achieved with a combination of field strength and pulse length that resulted in 50-75 % cell death . Limited data are available regarding the transformation efficiencies ob- tained with different plasmid DNAs and ...
... transformation were achieved with a combination of field strength and pulse length that resulted in 50-75 % cell death . Limited data are available regarding the transformation efficiencies ob- tained with different plasmid DNAs and ...
Page 1-76
... transformed at frequencies > 5 × 108 transformed colonies per microgram of supercoiled plasmid DNA . The maximum frequency of transformation that can be obtained routinely with most other strains of E. coli is approximately five- to ...
... transformed at frequencies > 5 × 108 transformed colonies per microgram of supercoiled plasmid DNA . The maximum frequency of transformation that can be obtained routinely with most other strains of E. coli is approximately five- to ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml