Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-49
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
... volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution , which is ...
Page 6-34
... volume during the first extraction , re - extract the organic phase once with 1/3 volume of TE ( pH 7.6 ) and combine the two aqueous phases . 4. Add 0.2 volume of 10 м ammonium acetate and 2 volumes of ethanol at 4 ° C . Store the ...
... volume during the first extraction , re - extract the organic phase once with 1/3 volume of TE ( pH 7.6 ) and combine the two aqueous phases . 4. Add 0.2 volume of 10 м ammonium acetate and 2 volumes of ethanol at 4 ° C . Store the ...
Page 7-7
... volumes of ice - cold PBS lacking calcium and magnesium ions ; use a wide- bore pipette to disperse the cell pellet gently , but completely , each time . b . Estimate the volume of the packed cells , and resuspend them in 10-20 volumes ...
... volumes of ice - cold PBS lacking calcium and magnesium ions ; use a wide- bore pipette to disperse the cell pellet gently , but completely , each time . b . Estimate the volume of the packed cells , and resuspend them in 10-20 volumes ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml