DNA ProfilingAustralian scholars of genetics, law, and agricultural biotechnology, present a handbook of DNA-based evidence for the legal, forensic, and law-enforcement professions. Explains to non-scientists how the genetic material in tissue residues is analyzed to provide direct identification of an individual. Describes the principles and procedures, the scientific aspects and legal implications of obtaining tissue samples, and problems that can arise in interpretation. Annotation copyrighted by Book News, Inc., Portland, OR |
Contents
THE SCIENCE | 12 |
Linear Backbone | 13 |
The | 26 |
Principles of DNA Profiling | 43 |
SPECIFIC METHODS OF DNA PROFILING | 51 |
Fixation of Transferred DNA Fragments to the Membrane | 58 |
CONCLUSIONS | 64 |
65 | |
The | 105 |
The Future | 127 |
Appendix A Technical Procedures | 141 |
Technical Procedures | 148 |
Technical Procedures | 174 |
Appendix F Technical Procedures | 183 |
Glossary | 192 |
199 | |
Common terms and phrases
alkali allele frequencies amount of DNA analysis autoradiographs B-globin bacterial bands base pairs base sequence blotting buffer cells centrifuge chromosome coincidental matching containing Court crime scene Crimes Act 1958 Criminal denatured detainee detected digestion discretion distilled water DNA duplex DNA fragments DNA probes DNA profiling DNA sequence Eco RI electrophoresis estimate ethanol ethidium bromide examination expert Figure Forensic Science fragment sizes gene genetic genome genotypes Hardy-Weinberg Hardy-Weinberg law human hybridization solution identification individual jury labeled probe laboratory Lifecodes linkage disequilibrium locus markers measurement minisatellite molecular multilocus nitrocellulose obtained particular plasmid police polymerase population frequencies probability probe DNA procedures Reagents refer to Chapter repeated residue restriction digestion restriction enzyme restriction fragments RFLP scientific sequence of bases specific specimen sperm stained standard strands suspect target DNA target sequence techniques temperature test gel tissue transfer tube usually variation VNTR VNTR loci VNTR probes volume X-ray film