Roger J. Miles, Robin Nicholas
Humana Press, 1998 - Medical - 330 pages
In Mycoplasma Protocols, Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenicity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products. Mycoplasma Protocols provides up-to-date and easy-to-follow mycoplasma methods for practical application in medical and veterinary diagnostic and research laboratories. The techniques permit effective work with these normally fastidious microorganisms, allowing investigators to illuminate their roles across a wide range of chronic respiratory, arthritic, and urogenital diseases. For all those working in this important field, Mycoplasma Protocols immediately becomes the methodological resource of choice.
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