Methods in Cell-Matrix Adhesion
Gulf Professional Publishing, Jun 14, 2002 - Science - 461 pages
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.
What people are saying - Write a review
We haven't found any reviews in the usual places.
acid activity adhesion assay afﬁnity alkanethiol analysis antibody atomic force microscopy basement membrane Biochem buffer cDNA cell adhesion Cell Biol Cell Biology cell lines cell surface cell–matrix chain Chem chromatography cloning coated collagen collagenase complex concentration containing culture deﬁned diluted dish domain ECM components embryos enzymes epithelial cells etal expression extracellular matrix ﬁbrin ﬁbronectin ﬁeld ﬁnal ﬁrst ﬂuorescence fragments function gene glycosaminoglycan growth factor heparan sulfate heparin heparin lyase Identiﬁcation images immunoprecipitation incubated inhibitors instructive ECM integrin interactions isolated keratinocytes kinase labeled laminin laminin-1 ligand ligand binding Mabs mammary matrix proteins medium mice microscopy migration modiﬁcation module molecular molecules monoclonal antibodies mutations Natl peptide perlecan plasmid plates polymerization proteoglycans protocols puriﬁcation receptor recombinant regulation reporter cells sample sequence signaling slices soluble solution speciﬁc structure substratum subunit target tenascin Timpl tissue transfected tube vitro vivo washed Yurchenco
Page 22 - O'Reilly MS. Boehm T., Shing Y., Fukai N., Vasios G., Lane WS, Flynn E.. Birkhead JR, Olsen BR, and Folkman J.
Page 22 - ... Fortini, ME, Li, PW, Apweiler, R., Fleischmann, W., Cherry, JM, Henikoff, S., Skupski, MP, Misra, S., Ashburner, M., Birney, E., Boguski, MS, Brody, T., Brokstein, P., Celniker, SE, Chervitz, SA, Coates, D., Cravchik, A., Gabrielian, A., Galle, RF, Gelbart, WM, George, RA, Goldstein, LS, Gong, F., Guan, P., Harris, NL, Hay, BA, Hoskins, RA, Li, J., Li, Z., Hynes, RO, Jones, SJ, Kuehl, PM, Lemaitre, B., Littleton, JT, Morrison, DK, Mungall, C., O'Farrell, PH, Pickeral, OK, Shue, C., Vosshall,...