Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 86
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) Po = 0 w2x 。 Pe + ... band center . Values of ẞo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
... band center at 1 atm pressure can be calculated from the approximate relationship of Eq . ( 69 ) Po = 0 w2x 。 Pe + ... band center . Values of ẞo for a range of useful gradient - forming salts and densities are given by Ifft et al ...
Page 89
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
... band of macro- solute and is determined from either schlieren or absorption data . In the case of a schlieren trace ( Fig . 30 ) , the center of the band is taken as the point of intersection of the biphasic trace due to the solute band ...
Page 267
... band overlap is carried out . Results obtained , for example , by Blout et al . ( 1961 ) , Brad- bury et al . ( 1967 ) , and Timasheff et al . ( 1967a ) show that spectra of a quality suitable for attempting analysis of band overlap can ...
... band overlap is carried out . Results obtained , for example , by Blout et al . ( 1961 ) , Brad- bury et al . ( 1967 ) , and Timasheff et al . ( 1967a ) show that spectra of a quality suitable for attempting analysis of band overlap can ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-¹ complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone