Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 11
... distribution of solute in which the concentration increases down the cell is established . The concentration distribution for a single solute is characterized by its molecular weight M1 , partial specific volume 1 , and nonideality , to ...
... distribution of solute in which the concentration increases down the cell is established . The concentration distribution for a single solute is characterized by its molecular weight M1 , partial specific volume 1 , and nonideality , to ...
Page 83
... distribution to give a distribution that is within . 1 % of the equilibrium concentration over two standard deviations about the band center . Equation ( 62 ) assumes that the equilibrium salt gradient is already established at zero ...
... distribution to give a distribution that is within . 1 % of the equilibrium concentration over two standard deviations about the band center . Equation ( 62 ) assumes that the equilibrium salt gradient is already established at zero ...
Page 89
... distribution of the macrosolute at equilibrium . With schlieren optics o is most simply obtained by directly measuring the distances of the maximum and minimum of the biphasic pattern , in a radial direction from the band center . In ...
... distribution of the macrosolute at equilibrium . With schlieren optics o is most simply obtained by directly measuring the distances of the maximum and minimum of the biphasic pattern , in a radial direction from the band center . In ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-¹ complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone