Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 340
... imidazole peaks of the four histidine residues . Peak 5 is a C - 4 imidazole resonance . The envelope labeled " aromatic " includes three other C - 4 imidazole peaks as well as peaks from six tyrosine and three phenylalanine residues ...
... imidazole peaks of the four histidine residues . Peak 5 is a C - 4 imidazole resonance . The envelope labeled " aromatic " includes three other C - 4 imidazole peaks as well as peaks from six tyrosine and three phenylalanine residues ...
Page 383
... imidazole groups in that protein . It is more realistic to consider a variation in individual pKa values with the progress of titration of the imidazole groups than to accept the full weight of the electrostatic term as such ...
... imidazole groups in that protein . It is more realistic to consider a variation in individual pKa values with the progress of titration of the imidazole groups than to accept the full weight of the electrostatic term as such ...
Page 420
... imidazole groups in the binding at final equilibrium is indicated by the sharp reduction of maximum copper ( II ) binding by the protein after treatment with iodoacetamide to alter the affinity of imidazole groups for the metal ion ...
... imidazole groups in the binding at final equilibrium is indicated by the sharp reduction of maximum copper ( II ) binding by the protein after treatment with iodoacetamide to alter the affinity of imidazole groups for the metal ion ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-ยน complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone