Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 129
... observed effect with increasing temperature can be either an increase in [ 7 ] ( usually observed in a poor solvent ) , no change , or a decrease in [ 7 ] ( usually observed with a good solvent ) , or the occur- rence of a flat maximum ...
... observed effect with increasing temperature can be either an increase in [ 7 ] ( usually observed in a poor solvent ) , no change , or a decrease in [ 7 ] ( usually observed with a good solvent ) , or the occur- rence of a flat maximum ...
Page 137
... observed also with globular proteins , providing that it is accompanied by an unfolding process . Of more current interest is the considerable increase in intrinsic viscosity which results from the fission of disulfide bonds in double ...
... observed also with globular proteins , providing that it is accompanied by an unfolding process . Of more current interest is the considerable increase in intrinsic viscosity which results from the fission of disulfide bonds in double ...
Page 292
... observed . Since coupling constants are independent of the applied field while chemical shifts are proportional to Ho , second - order fine structure is likely to be observed at low field strengths ( J ~ 8 ) , but spectra may become ...
... observed . Since coupling constants are independent of the applied field while chemical shifts are proportional to Ho , second - order fine structure is likely to be observed at low field strengths ( J ~ 8 ) , but spectra may become ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-¹ complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone