Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 89
... plot of buoyant density vs. activity of water and ( da1 ° / dp ° ) is the reciprocal of the slope of the plot of solu- tion density vs. water activity . Methods for the evaluation of these derivatives are given by Ifft and Vinograd ...
... plot of buoyant density vs. activity of water and ( da1 ° / dp ° ) is the reciprocal of the slope of the plot of solu- tion density vs. water activity . Methods for the evaluation of these derivatives are given by Ifft and Vinograd ...
Page 156
... plot equation . For this plot , the scattering is measured as a function of angle ( usually between 25 ° and 145 ° ) at different concentrations . The points are plotted as a function of [ C2 + sin2 ( 0/2 ) ] . Extrapolation to zero ...
... plot equation . For this plot , the scattering is measured as a function of angle ( usually between 25 ° and 145 ° ) at different concentrations . The points are plotted as a function of [ C2 + sin2 ( 0/2 ) ] . Extrapolation to zero ...
Page 175
... plot from 1 / M2 . When the charge is not too great and supporting electrolyte is present , the light - scattering plot is in the form of a straight line with a positive slope . The appropriate second virial coefficient has been derived ...
... plot from 1 / M2 . When the charge is not too great and supporting electrolyte is present , the light - scattering plot is in the form of a straight line with a positive slope . The appropriate second virial coefficient has been derived ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-¹ complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone