Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
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Page 11
... single solvent component . This will not be a valid assumption if one of the low molecular weight solutes binds preferentially to the protein , or if the low molecular weight solutes each . distribute very differently down the cell ...
... single solvent component . This will not be a valid assumption if one of the low molecular weight solutes binds preferentially to the protein , or if the low molecular weight solutes each . distribute very differently down the cell ...
Page 42
... single sharp peak does not indicate necessarily a single homogeneous protein . Broadly speaking the possibilities are : ( a ) a single protein , ( b ) two proteins with very similar sedimentation coefficients , ( c ) a major protein ...
... single sharp peak does not indicate necessarily a single homogeneous protein . Broadly speaking the possibilities are : ( a ) a single protein , ( b ) two proteins with very similar sedimentation coefficients , ( c ) a major protein ...
Page 63
... single equilibrium to form dimers , only a single asymmetric schlieren peak is seen . If the sys- tem is examined over a range of concentrations , the asymmetry may de- crease as concentration is increased . In the case of a solute in ...
... single equilibrium to form dimers , only a single asymmetric schlieren peak is seen . If the sys- tem is examined over a range of concentrations , the asymmetry may de- crease as concentration is increased . In the case of a solute in ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
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absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-ยน complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone