Physical Principles and Techniques of Protein Chemistry, Part 2Sydney J. Leach, Sidney J. Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals ... |
From inside the book
Results 1-3 of 88
Page 36
... values to standard conditions ( Section IV , B , 4 ) . Where sedimentation coefficient values are being extrap- olated to zero concentration , the viscosity and density of the solvent at the temperatures of the various experiments will ...
... values to standard conditions ( Section IV , B , 4 ) . Where sedimentation coefficient values are being extrap- olated to zero concentration , the viscosity and density of the solvent at the temperatures of the various experiments will ...
Page 77
... values of quantities are replaced by the value of these quantities at the center of sedimentation , indicated below by a subscript c 820 , w d ( ln x ) dt 32 720 , w an + D ... 1,2- ( 37 ) . D20 , w Xc nc ( 1 - upc ) 720 , w ( 1 Up20 ...
... values of quantities are replaced by the value of these quantities at the center of sedimentation , indicated below by a subscript c 820 , w d ( ln x ) dt 32 720 , w an + D ... 1,2- ( 37 ) . D20 , w Xc nc ( 1 - upc ) 720 , w ( 1 Up20 ...
Page 383
... values of n that yielded closely comparable values of w . Apply- ing Eq . ( 2 ) , pKa was read off at the point corresponding to Z equal to 0 . Because of restrictions on the range of pH in which the protein is stable , experimental values ...
... values of n that yielded closely comparable values of w . Apply- ing Eq . ( 2 ) , pKa was read off at the point corresponding to Z equal to 0 . Because of restrictions on the range of pH in which the protein is stable , experimental values ...
Contents
Ultracentrifugal Analysis J H Coates | 1 |
Glossary of Symbols | 2 |
Introduction | 3 |
Copyright | |
48 other sections not shown
Other editions - View all
Common terms and phrases
absorption acid amino anions atoms axial ratio band beam binding Biol bond Bradbury calculated capillary cell centrifugal chain changes Chem chemical shifts cm-ยน complex component concentration constant copper(II denaturation density gradient dependence determined dilution Doty effect electron ellipsoid enzyme equation extrapolation field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky length light scattering light-scattering lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin nuclei observed obtained optical density optical system partial specific volume particle PBLG peak peptide Phys plot Polymer Sci Proc protein solution protons random coil Rayleigh reference refractive index relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient sedimentation equilibrium shearing stress slit solvent spectra spectrum speed structure studies Tanford technique temperature Timasheff tion transition ultracentrifuge values velocity Vinograd viscometer zero zone