Manual of Methods for General Bacteriology |
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Page 357
Standard NH4 + solution : Prepare a stock NH4 + solution by dissolving 381 . ...
Reagent for removing trace amounts of interfering cations : Dissolve 50 g of Na
EDTA ( ethylenediaminetetraacetic acid , disodium salt ) in 60 ml of ammonium ...
Standard NH4 + solution : Prepare a stock NH4 + solution by dissolving 381 . ...
Reagent for removing trace amounts of interfering cations : Dissolve 50 g of Na
EDTA ( ethylenediaminetetraacetic acid , disodium salt ) in 60 ml of ammonium ...
Page 436
Boil to dissolve agar , dispense into tubes , and sterilize by autoclaving . Cool
tubes in a slanted position . any tubes that develop a light pink color ( indicating
oxidation ) upon standing . 20 . 3 . 29 . Seawater , Artificial 27 . 5 g 5 . 0 g 20 . 3 .
27 .
Boil to dissolve agar , dispense into tubes , and sterilize by autoclaving . Cool
tubes in a slanted position . any tubes that develop a light pink color ( indicating
oxidation ) upon standing . 20 . 3 . 29 . Seawater , Artificial 27 . 5 g 5 . 0 g 20 . 3 .
27 .
Page 438
0 ml Dissolve the NAD in the water . Store in a ... 20 ml Dissolve the aldehyde in
the ethanol and then add the acid . Protect from ... Add 20 pellets of NaOH with
stirring until the pellets are dissolved and the fumaric acid is in solution . Adjust
pH ...
0 ml Dissolve the NAD in the water . Store in a ... 20 ml Dissolve the aldehyde in
the ethanol and then add the acid . Protect from ... Add 20 pellets of NaOH with
stirring until the pellets are dissolved and the fumaric acid is in solution . Adjust
pH ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York