Manual of Methods for General Bacteriology |
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Page 31
... Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt ...
... Wash the slide several times in a solution consisting of 2 g of potassium metabisulfite and 5 ml of concentrated hydrochloric acid in 500 ml of distilled water . 8. Wash the slide with tap water , and counter- stain with a 0.002 % ( wt ...
Page 331
... Wash the filter in a hood with 2- to 5 - ml volumes of ethanol - diethyl ether ( 1 : 1 , vol / vol ) warmed to 45 ° C in a water bath by allowing the solvents to pass slowly through the filter ( 10 min ) . Collect the filtrate in a test ...
... Wash the filter in a hood with 2- to 5 - ml volumes of ethanol - diethyl ether ( 1 : 1 , vol / vol ) warmed to 45 ° C in a water bath by allowing the solvents to pass slowly through the filter ( 10 min ) . Collect the filtrate in a test ...
Page 468
... Wash membrane filter ( DNA duplexes remain on it ) Dry membrane filter and measure radioactivity FIG . 10. Scheme ... Wash in 2x SSC at the incubation temperature . Use two 300 - ml volumes of washing buffer , and wash for 5 min in each ...
... Wash membrane filter ( DNA duplexes remain on it ) Dry membrane filter and measure radioactivity FIG . 10. Scheme ... Wash in 2x SSC at the incubation temperature . Use two 300 - ml volumes of washing buffer , and wash for 5 min in each ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract