Manual of Methods for General Bacteriology |
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Page 311
The amount of unknown is derived from the equation : wt of unknown ( g ) same
ury to kno of the instance , 14C - sterol of known total activity can be added .
Determination of total 4C radioactivity of the pure isolated compound measures
the ...
The amount of unknown is derived from the equation : wt of unknown ( g ) same
ury to kno of the instance , 14C - sterol of known total activity can be added .
Determination of total 4C radioactivity of the pure isolated compound measures
the ...
Page 336
Determine the amount of total hexosamine in each sample from a standard curve
prepared by plotting the absorbance at 585 nm versus the amount of hexosamine
in each standard ( 16 . 1 . 1 ) . Glucosamine , galactosamine , and muramic ...
Determine the amount of total hexosamine in each sample from a standard curve
prepared by plotting the absorbance at 585 nm versus the amount of hexosamine
in each standard ( 16 . 1 . 1 ) . Glucosamine , galactosamine , and muramic ...
Page 464
Cool in a refrigerator for 1 h . to warm to room temperature before dispensing the
10 - ul amounts . ... ( which has no homology with bacterial DNA ) into two control
vials for measuring the amount of selfrenaturation of the labeled fragments .
Cool in a refrigerator for 1 h . to warm to room temperature before dispensing the
10 - ul amounts . ... ( which has no homology with bacterial DNA ) into two control
vials for measuring the amount of selfrenaturation of the labeled fragments .
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York