Manual of Methods for General Bacteriology |
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Page 67
BUFFERS 67 pH readings with tris ( hydroxymethyl ) amino- varies widely with the types of organic molecules methane ... Consider In instances where precision is not required , the following in selecting a buffer to use : such as in ...
BUFFERS 67 pH readings with tris ( hydroxymethyl ) amino- varies widely with the types of organic molecules methane ... Consider In instances where precision is not required , the following in selecting a buffer to use : such as in ...
Page 68
For example , to prepare 100 ml of a 0.1 M sodium phosphate buffer at pH 7.0 , mix solutions of 0.1 M NaH2PO4 ... The most commonly used buffers in bacterial growth media are phosphate , Tris - hydrochloride , citrate , and acetate .
For example , to prepare 100 ml of a 0.1 M sodium phosphate buffer at pH 7.0 , mix solutions of 0.1 M NaH2PO4 ... The most commonly used buffers in bacterial growth media are phosphate , Tris - hydrochloride , citrate , and acetate .
Page 461
Nicked DNA Tris buffer ( 2 - amino - 2 - hydroxymethyl1,3 - propanediol ) , 0.7 M , pH 7.8 MgCl2 , 0.14 M Dithioerythritol , 0.01 M ( Sigma Chemical Co. , St. Louis , Mo. ) dXTP solution - contains the following deoxynucleoside ...
Nicked DNA Tris buffer ( 2 - amino - 2 - hydroxymethyl1,3 - propanediol ) , 0.7 M , pH 7.8 MgCl2 , 0.14 M Dithioerythritol , 0.01 M ( Sigma Chemical Co. , St. Louis , Mo. ) dXTP solution - contains the following deoxynucleoside ...
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Contents
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
GROWTH RALPH N CoStilow Editor | 65 |
Copyright | |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method microscopy mixture mutants needed obtained organisms oxygen plasmid plate positive prepared present Press procedure protein reaction reagent references remove sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Author | Philipp Gerhardt |
| Editor | Philipp Gerhardt |
| Contributors | R. G. E. Murray, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826298, 9780914826293 |
| Length | 524 pages |
| Export Citation | BiBTeX EndNote RefMan |