Manual of Methods for General Bacteriology |
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Page 174
... centrifuge refrig- eration system to cool the head . If this is done , the centrifuge rotor will contract and seize the spindle , making it very difficult to remove at a later time . 3. Sterilize several capped centrifuge tubes ...
... centrifuge refrig- eration system to cool the head . If this is done , the centrifuge rotor will contract and seize the spindle , making it very difficult to remove at a later time . 3. Sterilize several capped centrifuge tubes ...
Page 236
... Centrifuge the mixture at 48,000 × g for 1 h to pellet the phage . Gently resuspend the pellet in 0.1 volume of L broth containing 2 mM ethylenediaminetetraacetate and 10 mM CaCl2 . 11. Centrifuge at 15,000 × g for 10 min to remove ...
... Centrifuge the mixture at 48,000 × g for 1 h to pellet the phage . Gently resuspend the pellet in 0.1 volume of L broth containing 2 mM ethylenediaminetetraacetate and 10 mM CaCl2 . 11. Centrifuge at 15,000 × g for 10 min to remove ...
Page 344
Philipp Gerhardt. Centrifuge : A refrigerated centrifuge capable of achieving 10,000 × g Pyrex glass boiling tubes Volumetric flask : 10 ml Boiling - water bath Reagent - grade sulfuric acid : The quality of the sulfuric acid is ...
Philipp Gerhardt. Centrifuge : A refrigerated centrifuge capable of achieving 10,000 × g Pyrex glass boiling tubes Volumetric flask : 10 ml Boiling - water bath Reagent - grade sulfuric acid : The quality of the sulfuric acid is ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract