Manual of Methods for General Bacteriology |
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Page 146
... culture : 1. Solid culture is limited in the scale to which it can be increased ( e.g. , one can effectively produce gram amounts of cells , decigram amounts become difficult , and centigram or kil- ogram amounts are impossible in the ...
... culture : 1. Solid culture is limited in the scale to which it can be increased ( e.g. , one can effectively produce gram amounts of cells , decigram amounts become difficult , and centigram or kil- ogram amounts are impossible in the ...
Page 382
... culture is also made anaerobic . The accumulation and subse- quent utilization of NO2 in the culture medium can also be measured as an indicator of regula- tion over activity of the nitrate ( and nitrite ) reductase system . With a ...
... culture is also made anaerobic . The accumulation and subse- quent utilization of NO2 in the culture medium can also be measured as an indicator of regula- tion over activity of the nitrate ( and nitrite ) reductase system . With a ...
Page 384
... culture , add the glucose at approximately 150 min after IPTG ( isopropyl - B - D - thiogalactopyranoside ) addition . CAMP is solubilized by neutralization with NaOH . In this culture , add the cAMP about 150 min after IPTG addition ...
... culture , add the glucose at approximately 150 min after IPTG ( isopropyl - B - D - thiogalactopyranoside ) addition . CAMP is solubilized by neutralization with NaOH . In this culture , add the cAMP about 150 min after IPTG addition ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract