Manual of Methods for General Bacteriology |
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Page 231
After 2 or 3 reversion promoted by several commonly used days of incubation at
37°C , determine the relachemical ... revertants from partial revertants or
suppressed and NTG , which has a specificity spectrum simstrains by
determining ...
After 2 or 3 reversion promoted by several commonly used days of incubation at
37°C , determine the relachemical ... revertants from partial revertants or
suppressed and NTG , which has a specificity spectrum simstrains by
determining ...
Page 246
Analyze the results to determine whether the total number of transformants
increases proportionally with increases in the amount of donor DNA used . This
would be expected except at high concentrations of DNA when saturation may be
...
Analyze the results to determine whether the total number of transformants
increases proportionally with increases in the amount of donor DNA used . This
would be expected except at high concentrations of DNA when saturation may be
...
Page 267
In some cases , genetic methods are not avail - able so that one must directly
examine a bacte - rium to determine its plasmid content . Such an analysis
usually can be performed to determine simply whether a plasmid is or is not
present .
In some cases , genetic methods are not avail - able so that one must directly
examine a bacte - rium to determine its plasmid content . Such an analysis
usually can be performed to determine simply whether a plasmid is or is not
present .
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York