Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 246
Analyze the results to determine whether the total number of transformants
increases proportionally with increases in the amount of donor DNA used. This
would be expected except at high concentrations of DNA when saturation may be
...
Analyze the results to determine whether the total number of transformants
increases proportionally with increases in the amount of donor DNA used. This
would be expected except at high concentrations of DNA when saturation may be
...
Page 267
In some cases, genetic methods are not available so that one must directly
examine a bacterium to determine its plasmid content. Such an analysis usually
can be performed to determine simply whether a plasmid is or is not present.
In some cases, genetic methods are not available so that one must directly
examine a bacterium to determine its plasmid content. Such an analysis usually
can be performed to determine simply whether a plasmid is or is not present.
Page 332
Determine the radioactivity of the dried protein by use of a toluene-based
scintillation cocktail (see 16.4.3). 17.1.8. Applications The techniques described
above are usually applied to the fractionation of bacterial cells labeled with a
medium ...
Determine the radioactivity of the dried protein by use of a toluene-based
scintillation cocktail (see 16.4.3). 17.1.8. Applications The techniques described
above are usually applied to the fractionation of bacterial cells labeled with a
medium ...
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Contents
Electron Microscopy Roger M Cole and Terry J Popkin | 34 |
Cell Fractionation Carl A Schnaitman | 52 |
GROWTH Ralph N Costilow Editor | 65 |
Copyright | |
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose supernatant surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Bacteriological technics Bacteriology Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |