Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
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Page 371
GENERAL CONSIDERATIONS 371 Competing side reactions and use of
coupled enzyme assays. ... These involve the coupling of a primary enzyme (the
enzyme whose activity is being measured) system to one or two auxiliary
enzymes ...
GENERAL CONSIDERATIONS 371 Competing side reactions and use of
coupled enzyme assays. ... These involve the coupling of a primary enzyme (the
enzyme whose activity is being measured) system to one or two auxiliary
enzymes ...
Page 372
larger amounts of the auxiliary enzyme until the linear initial velocity of the
primary reaction can be determined accurately. A procedure developed and
described by McClure (29) is available for determining the time required to
produce this ...
larger amounts of the auxiliary enzyme until the linear initial velocity of the
primary reaction can be determined accurately. A procedure developed and
described by McClure (29) is available for determining the time required to
produce this ...
Page 379
The ability of L-isoleucine to allosterically inhibit this enzyme can be
demonstrated by performing the following experiment (H. E. Umbarger, personal
communication). Prepare tubes containing the components shown in Table 1.
Incubate at 37 ...
The ability of L-isoleucine to allosterically inhibit this enzyme can be
demonstrated by performing the following experiment (H. E. Umbarger, personal
communication). Prepare tubes containing the components shown in Table 1.
Incubate at 37 ...
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Contents
Electron Microscopy Roger M Cole and Terry J Popkin | 34 |
Cell Fractionation Carl A Schnaitman | 52 |
GROWTH Ralph N Costilow Editor | 65 |
Copyright | |
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Common terms and phrases
acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction g Distilled glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen Pasteur pipette pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove riboflavin salts sample slide sodium solution specific staining standard sterile substrate sucrose supernatant surface suspension Table techniques temperature thiamine tion tube vials vitamin vol/vol volume Wash wt/vol yeast extract
Bibliographic information
| Title | Manual of methods for general bacteriology: by Philipp Gerhardt, editor-in-chief ; R.G.E. Murray, editor, I. Morphology ... [et al.]. |
| Author | American Society for Microbiology |
| Editors | Philipp Gerhardt, American Society for Microbiology |
| Edition | illustrated, reprint |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › › Bacteriological technics Bacteriology Science / General Science / Life Sciences / Microbiology |
| Export Citation | BiBTeX EndNote RefMan |