Manual of Methods for General Bacteriology |
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Page 372
larger amounts of the auxiliary enzyme until the linear initial velocity of the
primary reaction can be determined accurately . A procedure developed and
described by McClure ( 29 ) is available for determining the time required to
produce this ...
larger amounts of the auxiliary enzyme until the linear initial velocity of the
primary reaction can be determined accurately . A procedure developed and
described by McClure ( 29 ) is available for determining the time required to
produce this ...
Page 373
OXIDOREDUCTASES 373 Enzyme activity versus time . In duplicate , prepare 10
to 20 enzyme reaction mixtures complete with a suitable mass of cells ( a few
preliminary trials with different dilutions of cells will generally be needed ) .
OXIDOREDUCTASES 373 Enzyme activity versus time . In duplicate , prepare 10
to 20 enzyme reaction mixtures complete with a suitable mass of cells ( a few
preliminary trials with different dilutions of cells will generally be needed ) .
Page 378
General Considerations When an accurate and sensitive assay is available and
has been standardized , one can proceed to determine whether that enzyme is
subject to one or more of several metabolic control pro - cesses . Two major types
...
General Considerations When an accurate and sensitive assay is available and
has been standardized , one can proceed to determine whether that enzyme is
subject to one or more of several metabolic control pro - cesses . Two major types
...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
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Common terms and phrases
absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York
Bibliographic information
| Title | Manual of Methods for General Bacteriology |
| Authors | Philipp Gerhardt, American Society for Microbiology |
| Editor | Philipp Gerhardt |
| Edition | illustrated |
| Publisher | American Society for Microbiology, 1981 |
| ISBN | 0914826301, 9780914826309 |
| Length | 524 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |