Manual of Methods for General Bacteriology |
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Page 172
Detailed description of methods for immobi - lized - cell reactors is perhaps
beyond the scope of this book , and the reader is referred to recent reviews on
the subject ( 6 , 7 , 13 ) . posite of a filter support ( often filter paper or cloth of
relatively ...
Detailed description of methods for immobi - lized - cell reactors is perhaps
beyond the scope of this book , and the reader is referred to recent reviews on
the subject ( 6 , 7 , 13 ) . posite of a filter support ( often filter paper or cloth of
relatively ...
Page 331
Wash the filter with 3 ml of ice - cold 70 % ethanol to remove residual
trichloroacetic acid , which may cause solubili - zation of some protein in the next
step . Discard the filtrate . 3 . Place the filter holder ( with the filter and suction
flask ) and a ...
Wash the filter with 3 ml of ice - cold 70 % ethanol to remove residual
trichloroacetic acid , which may cause solubili - zation of some protein in the next
step . Discard the filtrate . 3 . Place the filter holder ( with the filter and suction
flask ) and a ...
Page 494
Provide protection against pulling bacterial aerosols or overflow fluid into the
vacuum system by using an air filter in the line leading to the vacuum source and
using an overflow flask for liquids between the collection flask and the air filter .
Provide protection against pulling bacterial aerosols or overflow fluid into the
vacuum system by using an air filter in the line leading to the vacuum source and
using an overflow flask for liquids between the collection flask and the air filter .
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York