Manual of Methods for General Bacteriology |
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Page 60
... fragments to aggregate or fuse into larger membrane fragments which can then be sedimented ( 6 ) . In some cases , this non - sedi- mentable membrane fraction can represent as much as 20 to 30 % of the cytoplasmic membrane . Its ...
... fragments to aggregate or fuse into larger membrane fragments which can then be sedimented ( 6 ) . In some cases , this non - sedi- mentable membrane fraction can represent as much as 20 to 30 % of the cytoplasmic membrane . Its ...
Page 271
... fragment patterns which depend upon the number and spacing of the specific recognition sites within the genome . The DNA fragments can be separated by electrophoresis in agarose gels . Comparison of the patterns ob- tained for different ...
... fragment patterns which depend upon the number and spacing of the specific recognition sites within the genome . The DNA fragments can be separated by electrophoresis in agarose gels . Comparison of the patterns ob- tained for different ...
Page 272
... fragments that have been separated on agarose gels is highly desirable . These fragments can be useful for physical and ... fragment can be processed simultaneously . 2. Crush a gel slice in 0.1 M Tris at pH 5.95 ( 0.1 ml per gel slice ) ...
... fragments that have been separated on agarose gels is highly desirable . These fragments can be useful for physical and ... fragment can be processed simultaneously . 2. Crush a gel slice in 0.1 M Tris at pH 5.95 ( 0.1 ml per gel slice ) ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract