Manual of Methods for General Bacteriology |
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Page 10
... glass plates facing each other across a precisely measured gap , and Wratten filters ( Kodak ) consist of colored gel- atin mounted between two 5 - cm - square flats of optically polished glass . Wratten filters deterio- rate with time ...
... glass plates facing each other across a precisely measured gap , and Wratten filters ( Kodak ) consist of colored gel- atin mounted between two 5 - cm - square flats of optically polished glass . Wratten filters deterio- rate with time ...
Page 340
... glass dish . Repeat the acid treatment and water wash . Apply a vacuum to the resin in the funnel to remove the water , and add to the resin in succession : 150 ml of methanol , 150 ml of methanol - chloroform ( 1 : 1 , vol / vol ) ...
... glass dish . Repeat the acid treatment and water wash . Apply a vacuum to the resin in the funnel to remove the water , and add to the resin in succession : 150 ml of methanol , 150 ml of methanol - chloroform ( 1 : 1 , vol / vol ) ...
Page 341
... glass - stoppered tubes . Distribute each phospholipid into three equal portions in two glass - stoppered 15 - ml test tubes and a lyophile ampoule for further analysis . Evaporate the sol- vents at 37 ° C under a stream of nitrogen ...
... glass - stoppered tubes . Distribute each phospholipid into three equal portions in two glass - stoppered 15 - ml test tubes and a lyophile ampoule for further analysis . Evaporate the sol- vents at 37 ° C under a stream of nitrogen ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract