Manual of Methods for General Bacteriology |
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Page 294
With excess glucose oxidase and limiting glucose in the sample , the potentiomet
. ric response measures glucose in the sample . With excess glucose and limiting
glucose oxidase , the rate of potential change can be used to determine the ...
With excess glucose oxidase and limiting glucose in the sample , the potentiomet
. ric response measures glucose in the sample . With excess glucose and limiting
glucose oxidase , the rate of potential change can be used to determine the ...
Page 334
Commercial glucose oxidase - peroxidase preparation : Prepare the dry enzyme
mixture according to the manufacturer ' s instructions . Chromogen : Available as
part of the commercial kits . Prepare according to the manufacturer ' s ...
Commercial glucose oxidase - peroxidase preparation : Prepare the dry enzyme
mixture according to the manufacturer ' s instructions . Chromogen : Available as
part of the commercial kits . Prepare according to the manufacturer ' s ...
Page 382
02 M glucose and 0 . 25 % ( final concentrations ) vitamin - free casein
hydrolysate just prior to in - oculation . Let the cultures grow with vigorous
shaking to approximately 50 ug of cell dry weight per ml of culture or longer (
visible turbidity ) ...
02 M glucose and 0 . 25 % ( final concentrations ) vitamin - free casein
hydrolysate just prior to in - oculation . Let the cultures grow with vigorous
shaking to approximately 50 ug of cell dry weight per ml of culture or longer (
visible turbidity ) ...
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Contents
55 | 32 |
Electron Microscopy ROGER M COLE AND TERRY J POPKIN | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
Copyright | |
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absorbance acid activity added addition Adjust agar allow amino acids amount anaerobic applications appropriate assay autoclaving bacteria Bacteriol base broth buffer cells centrifuge colonies column components compounds concentration containing counting cover culture described determine dilution Dissolve distilled water effective electron enrichment enzyme examine example extract filter flask fraction give glass glucose grow growth heat Incubate inoculated isolation laboratory light liquid liter material measure medium membrane method mg/liter microscopy mixture mutants obtained organisms oxygen plasmid plates positive prepared present Press procedure protein reaction reagent references remove salts sample selection separation slide sodium solution specific staining standard sterile substrate surface suspension Table techniques temperature tion transfer tube usually values vitamin volume Wash York