Manual of Methods for General Bacteriology |
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Page 453
... Hydroxylapatite Method Britten et al . ( 13 ) were the first to isolate DNA by adsorbing it to hydroxylapatite . Since then , several modifications of the method have been described ( 32 , 35 , 43 , 47 ) . The following is a useful ...
... Hydroxylapatite Method Britten et al . ( 13 ) were the first to isolate DNA by adsorbing it to hydroxylapatite . Since then , several modifications of the method have been described ( 32 , 35 , 43 , 47 ) . The following is a useful ...
Page 455
... hydroxylapatite procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to ...
... hydroxylapatite procedure is that the RNA species are degraded to such an extent that they will not compete with DNA for adsorption sites on the hydroxyl- apatite . For some groups of organisms , RNase T1 may be required in addition to ...
Page 464
... hydroxylapatite or the S1 nuclease procedure ( see below ) . Hydroxylapatite procedure . The hydroxylapatite procedure separates dou- ble - stranded DNA from single - stranded DNA by selective adsorption to hydroxylapatite ( Bio- Rad ...
... hydroxylapatite or the S1 nuclease procedure ( see below ) . Hydroxylapatite procedure . The hydroxylapatite procedure separates dou- ble - stranded DNA from single - stranded DNA by selective adsorption to hydroxylapatite ( Bio- Rad ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract