Manual of Methods for General Bacteriology |
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Page 187
... incubated , examine the distribution of colonies on all of the plates to learn how uniform your spreading tech- nique has become . Those that have a larger number of colonies are especially useful in this regard , even if they have too ...
... incubated , examine the distribution of colonies on all of the plates to learn how uniform your spreading tech- nique has become . Those that have a larger number of colonies are especially useful in this regard , even if they have too ...
Page 252
... incubated at 30 ° C . As the last step , pick individual isolated colonies from each trans- ductant into 2 ml of ... incubating at 37 or 42 ° C . As a control , test for growth at 30 ° C as well . 2. The transductants should be immune to ...
... incubated at 30 ° C . As the last step , pick individual isolated colonies from each trans- ductant into 2 ml of ... incubating at 37 or 42 ° C . As a control , test for growth at 30 ° C as well . 2. The transductants should be immune to ...
Page 396
... incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid may require ...
... incubation times . 5. Recentrifugation . After each mixture of pellet and test solution has been incubated , it should be centrifuged sufficiently to obtain a cell - free supernatant fluid for assay . The supernatant fluid may require ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract