Manual of Methods for General Bacteriology |
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Page 60
... membrane is insoluble in Triton X - 100 while the cytoplas- mic membrane is completely solubilized . When cells are broken by shear forces , some of the cytoplasmic membrane becomes frag- mented extensively ( probably into short ...
... membrane is insoluble in Triton X - 100 while the cytoplas- mic membrane is completely solubilized . When cells are broken by shear forces , some of the cytoplasmic membrane becomes frag- mented extensively ( probably into short ...
Page 459
... membrane filters . The top and bottom pieces are shown . Five filters are present in the right - hand side of the bottom piece ( see also Fig . 5 ) . ( D ) Punch for cutting oblong membrane filters . ( E ) Automatic micropipette with ...
... membrane filters . The top and bottom pieces are shown . Five filters are present in the right - hand side of the bottom piece ( see also Fig . 5 ) . ( D ) Punch for cutting oblong membrane filters . ( E ) Automatic micropipette with ...
Page 468
... membranes ( about 5 με DNA per membrane ) from a 15 - cm membrane with a keysort punch ( 22.6.1 ) . 2. Preincubate the membranes at the incu- bation temperature of the experiment for 0.5 to 2 h . Agitate the membranes several times ...
... membranes ( about 5 με DNA per membrane ) from a 15 - cm membrane with a keysort punch ( 22.6.1 ) . 2. Preincubate the membranes at the incu- bation temperature of the experiment for 0.5 to 2 h . Agitate the membranes several times ...
Contents
Determinative Methods of Light Microscopy R N DOETSCH | 34 |
Cell Fractionation CARL A SCHNAITMAN | 52 |
RALPH N COSTILOW Editor | 63 |
Copyright | |
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acetate Add per liter Adjust agar amino acids anaerobic aqueous assay autoclaving bacteria bacterial cells Bacteriol bacterium biotin broth buffer calcium pantothenate carbon centrifuge chemical coli colonies column components compounds concentration containing cover slip culture described determine dilution Dissolve distilled water electron microscopy endospores enrichment enzyme Escherichia coli ethanol film filter filtration flask folic acid fraction glass glucose gradient gram-negative grid growth heat hydroxylapatite Incubate inoculated isolation laboratory lipids liquid measure medium membrane method mg/liter Microbiol microbiology mixture mutagens mutants NaCl nitrogen nutrient organisms oxidation oxygen pellet phage pipette plasmid plate prepared procedure protein reaction reagent remove salts sample slide sodium solution specific specimen staining standard sterile substrate sucrose surface suspension Table techniques temperature thiamine tion tube vials vitamin vitamin B12 vol/vol volume Wash wt/vol yeast extract